EN
Whole Genome Sequencing

Human whole genome sequencing enables researchers to catalog the genetic constitution of individuals and capture all the variants present in a single assay. It is applied to the study of cancer and a variety of diseases, as well as human population evolution studies and pharmacogenomics, which has great research and industrial value.


Technical route

Sequencing Strategy
1.HiSeq X platform, paired-end 150 bp
2.Recommended Sequencing Depth:30~50X

Sample Requirements
1.DNA amount: ≥ 2 μg
2.DNA concentration: ≥ 20 ng/μl
3.Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination

Turnaround Time
35 working days

List of Analyses
1.Data quality control
2.Alignment with reference genome, statistics of sequencing depth and coverage
3.SNP/InDel/SV/CNV calling, annotation and statistics
4.Somatic SNP/InDel/SV/CNV calling, annotation and statistics (paired tumor samples)