EN
Animal & Plant Genome Resequencing

Whole genome sequencing refers to the existing reference genome species genome sequencing, can be used to analyze differences in individual and group level. Through sequence alignment, site and structure of the difference in their comprehensive mining sequences variation. Whole genome sequencing has become one of the most rapid and effective method of molecular breeding and evolution.


Sequencing Strategy
1.HiSeq X platform, paired-end 150 bp
2.Recommended Sequencing Depth:10~30X


Sample Requirements
1.DNA amount: ≥ 3 μg
2.DNA concentration: ≥ 30 ng/μl
3.Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination

Turnaround Time
35 working days

List of Analyses
1.Data quality control
2.Alignment with reference genome, statistics of sequencing depth and coverage
3.SNP/InDel/SV/CNV calling, annotation and statistics
4.Transposon detection, annotation and statistics


Application

Bin Map construction
BSA analysis
Genome-wide association study
Population evolution