EN
2b-RAD

2b - RAD is based on type II B restriction enzymes (Nature of the Methods (wang et al, 2012)). The technology overcomes some shortcomings of RAD-Seq and GBS. It provides 33-36 bp enzyme label, sequencing after label enrichment to achieve SNP screening and genotyping analysis from whole genome wide range.

Technical characteristics
1.Strong flexibility, tag number is under control
2.The label length is consistent, with a consistent PCR amplification efficiency
3.Position specificity in labels, does not depend on the reference sequence and assembly result
4.Codominant markers SNPS is available, also can make use of dominant markers
5.Based on mixed poisson distribution model of de novo SNP classification (iML), which can remove the distraction of repetitive sequence effectively. Classification accuracy is better than existing algorithms, with a false positive rate is lower 20%.

Application
Bin Map construction and QTL location
Population genetic study
Genome-wide association study
Population evolution analysis


technology flow


Technique Data
1.Genome label average density: 2kb
2.The average depth of lable ≥ 20X
3.Classification accuracy≥ 95%l

Sample Requirements
1.Species: Diploid organisms, not apply in polyploid species
2.DNA amount: ≥ 1 μg
3.DNA concentration: ≥ 200 ng/μl
4.Sample background: specific genetic relationship between construction group, Number of individuals ≥ 100

Turnaround Time
60 working days

List of Analyses
1.Data quality control
2.Get high quality label
3.Label clustering
4.SNP classification based on iML
5.Mark separation analysis
6.Bin Map construction

Customized information analysis
1.QTL location (need provide Phenotypic data)
2.Bin Map Integration (need provide original map data)
3.Genome-wide association study