EN
De Novo Bacterial Genome Sequencing

According to different research purpose and assembly level, we provide two products: Primary Assembly for bacteria, and Complete map for bacteria.The complete map of a bacterial genome provides researchers with the complete genome sequence. Based on assembly results, researchers can conduct detailed investigations of key genes and proteins to reveal their specific functions and predict molecular mechanisms. De novo genome sequencing can also be used to study the the pathogenicity of the pathogen, the prevention and treatment of disease.

Technical parameters

 

Primary Assembly

Complete Map

Sample Requirements

Genome DNA

Genome DNA

m≥2 μg,c≥50 ng/μL

m≥30 μg,c≥100 ng/μL

Sequencing platform

HiSeq PE150

PacBio, HiSeq and other platform

Sequencing Strategy

≥200X

350bp Insert ≥100X
10K Insert ≥100X

Library Strategy

350bp Insert

350bp Insert + 10K Insert

Technique target

Primary assembly

1 contig,0 gap

Turnaround time

30 working days

60 working days



List of Analyses

1.Data quality control
2.Denovo assembly
3.Genome Component analysis (Genomic island prediction, Repetitive sequence prediction, Non-coding RNA prediction, Gene prediction, CRISPR prediction, Prophage prediction)
4.Gene Function analysis(COG、KEGG、NR、GO、Swiss-prot)
5.Pathogenicity Analysis(Type III secretion system effector protein (Gram negative bacteria)、PHI、VFDB、ARDB、CAZy) 6.Comparative Genome Analysis(Synteny analysis、Core-Pan Gene Analysis、Specie Evolution analysis)