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Resequencing for bacteria&fungi

Resequencing for Bacteria&fungi genome mainly used to detect the differences between gene sequence and structure variation. That is, Detecting single nucleotide polymorphism (SNP), Insertion or deletion (Indel)、structure variation (SV) and other mutation types.

Sequencing Strategy
1.HiSeq platform, paired-end 150 bp
2.Recommended Sequencing Depth:100X

Sample Requirements
1.DNA amount: ≥ 2 μg
2.DNA concentration: ≥ 50 ng/μl
3.Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination

Turnaround Time
30 working days

List of Analyses

Resequencing for bacteria

Advanced Assembly

  1. 1. Data quality control
  2. 2.Primary assembly
  3. 3.Alignment with reference genome
  4. 4.SNP、Indel、SV calling, annotation and statistics
  5. 5.Species Evolution Analysis (phylogenetic analysis,Ka/Ks)
  1. 1.Data quality control
  2. 2.Alignment with reference genome
  3. 3.SNP、Indel\SV calling, annotation and statistics
  4. 4.Species Evolution Analysis (phylogenetic analysis,Ka/Ks)