Resequencing for Bacteria&fungi genome mainly used to detect the differences between gene sequence and structure variation. That is, Detecting single nucleotide polymorphism (SNP), Insertion or deletion (Indel)、structure variation (SV) and other mutation types.
1.HiSeq platform, paired-end 150 bp
2.Recommended Sequencing Depth:100X
1.DNA amount: ≥ 2 μg
2.DNA concentration: ≥ 50 ng/μl
3.Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination
30 working days
Resequencing for bacteria