EN
Transcriptomics

Transcriptomics including mRNA, miRNA, lncRNA, cirRNA, rRNA, tRNA, piRNA, etc. Through the transcriptome high-throughput sequencing technologies, provides expression quantity changes or variation structure(such as cSNP, alternative splicing, gene fusion) in different tissue and cell. It is widely used in disease research, drug response research, pharmacokinetics, and personalized healthcare reasearch.

Technical parameters


Sample type

Sequencing platform Library type Target RNA enrichment Recommend data Sequencing
Strategy

Turnaround 
time

DGE








Total RNA

m≥2 μg
c≥50 ng/μL
Transcriptome library Olig dT ≥3G clean data

HiSeq PE150

35 workdays

Eukaryotic
Transcriptome

m≥2 μg
c≥50 ng/μL
Transcriptome library Olig dT ≥8G clean data HiSeq PE150 35 workdays

Prokaryotic  
Transcriptome

m≥5 μg
c≥65 ng/μL
Transcriptome library / strand-specific library Ribozero kit remove rRNA ≥2G clean data (with ref)
≥3G clean data(without ref)
HiSeq PE150 35 workdays

miRNA

m≥3 μg
c≥200 ng/μL
Small RNA library / ≥10M reads HiSeq SE50 35 workdays

lncRNA

m≥4 μg
c≥120 ng/μL

strand-specific library
Ribozero kit remove rRNA ≥12G clean data HiSeq PE150 45 workdays



List of Analyses