Whole Genome Bisulfite Sequencing

DNA methylation is an important part of epigenetics, plays an important role in regulating gene expression, including gene regulation, stem cell differentiation, embryogenesis, aging, cancer and other diseases, and phenotypic diversity and evolution in plants and animals.

Sequencing Strategy
1.HiSeq platform, paired-end 150 bp
2.Recommended Sequencing Data: 5G clean data

Sample Requirements
DNA amount: ≥ 5 μg, DNA concentration: ≥ 100 ng/μl

Turnaround Time
50 working days

List of Analyses
1.Data quality control
2.Alignment with reference genome
3.Sequence Depth and Coverage Analysis
4.Calculation of Methylation Level
5.Global Trends of Methylome
6.Identification of Differentially Methylated Regions (DMRs)